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Figure 6


Fig. 6. Expression and subcellular localization of PIG-1. (A-F) Images of larvae expressing Ppig-1::gfp: anterior, left; ventral, up. (A,C,E) DIC microscopy; (B,D,F) GFP fluorescence. (A,B) L1 larva expressing Ppig-1::gfp in the Q neuroblast (arrow) and V5 seam cell (arrowhead). (C,D) L1 larva expressing Ppig-1::gfp in the Q neuroblast daughters Q.a (arrow) and Q.p (arrowhead). (E,F) L1 larva expressing Ppig-1::gfp in the Q lineage descendants AQR (asterisk), AVM (arrow), and SQDR (arrowhead). (G,H) DIC (G) and fluorescence (H) images of an embryo expressing Ppig-1::pig-1::gfp. pig-1::gfp is present throughout the cytoplasm and excluded from the nucleus. Scale bars: 10 µm.