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Figure 4


Fig. 4. Expression of Flk1, Tie2, CD31 or Oct3/4-GFP in E7.5 embryos. Representative flow cytometric analyses of pooled embryonic cells around E7.5 are shown. (A) Cells stained with an anti-Flk1 antibody. 6.0±11.1% (mean±s.d., n=9) of the cells showed expression of Flk1. (B) Cells stained with an anti-Tie2 antibody. 3.0±2.7% (mean±s.d., n=4) of the cells showed expression of Tie2. (C) Cells stained with an anti-CD31 antibody. 4.3±3.8% (mean±s.d., n=5) of the cells showed expression of CD31. (D) 39.0±25.3% (mean±s.d., n=6) of the cells from Oct3/4-GFP+/- embryos expressed GFP.