Fig. 4. Cerebellar anlage abnormalities in mouse embryos with ectopic expression of Lmx1a. (A-F). Dorsal view of E12.5 whole embryos (A,B) and their dissected neural tubes (C-F). Arrows indicate dramatically expanded medial single cell layer in transgenics (B,D,F). (G-W) Paramedial sagittal sections of E12.5 cerebella with genotypes and markers indicated, together with quantification of each cell type. In all panels, insets show a higher magnification of the boxed regions. Roof plate (arrow in H showing Gdf7 and arrows in J showing Lmx1a⁺/Msx1/2⁺ cells) was expanded to the ventricular surface within the anlage. Arrowheads in I and J point to Lmx1a^-/Msx1/2⁺ cells located adjacent to Lmx1a⁺/Msx1/2⁺ roof plate cells. (K,N,Q,T,W) Quantification of number of c1, RLS, pc2 and c2-c4 cells in Lmx1a transgenic embryos. ^* indicates P<0.01. Bars indicate s.e.m.