Fig. 4. Ci-sFRP1/5 is a target of Ci-FoxA-a via the sFRP-118 element. (A) Weak expression of Ci-FoxA-a by whole-mount in situ hybridization at the eight-cell stage in the anterior animal (a4.2) and vegetal (A4.1) lineages. Lateral view, anterior towards the left, animal towards the top. The staining is detected in the nuclear region. (B-E) Effect of the down- or upregulation of Ci-FoxA-a on sFRP-118 activity at the 110-cell stage (histogram in centre is colour coded according to the lineage) and on endogenous Ci-sFRP1/5 expression at the 64-cell and neurula stages (right). (B,C) Embryos microinjected with control or Ci-FoxA-a morpholinos. The percentage of embryos with a-line staining is indicated. (D) Control and (E) embryos electroporated with the indicated Ci-FoxA-a overexpression construct. The percentage of embryos with ectopic b-line staining is indicated. Black arrows indicate ectopic posterior ectodermal expression. Anterior is towards the top, animal views are shown at the 64-cell stage, vegetal views are shown at the neural plate stage.