Fig. 5. Ci-Fox-A-a activates anterior ectodermal markers. (A-F) Effect of pan-animal Ci-FoxA-a overexpression on the neural plate-stage activity of the sFRP-118 enhancer, visualized by X-gal staining. (A,B) Whole embryos; (C,D) anterior ectodermal explants cut at the eight-cell stage and cultured in isolation; (E,F) posterior ectodermal explants. The percentage indicates the proportion of stained explants. Whole embryos are shown in animal views at the neural plate stage. (G-O) Effect of pan-animal overexpression or knock down of Ci-FoxA-a on Ci-Otx (G-I), Ci-FoxC (J,K) and Ci-Ror-a (L-N). All embryos are shown in vegetal view at the neural plate stage, anterior towards the top. The percentages of embryos with ectopic expression in b-line cells are indicated. (O) Schematic vegetal view of the neural plate showing the position of anterior (a9.) and posterior (b9.) ectodermal lineages at neural plate stage. Anterior is towards the top.