Fig. 6. MAK inhibits Wnt signaling. (A,B) Axis-inducing activity of Dsh is suppressed by MAK, but not MAK-KD RNA. (A) Indicated RNAs (2 ng) were injected into a single ventral cell of four-cell embryos and secondary axes were assessed at stages 35-38. (B) Summary of axis induction assays, demonstrating that MAK, but not MAK-KD, inhibits Dsh signaling. Partial axes contained secondary neural tubes, usually up to the hindbrain level, without eyes, complete secondary axes contained eyes and/or cement glands. (C) MAK inhibits pSiaLuc reporter activation induced by Xwnt8 and Dsh, but not by ß-catenin. Luciferase activity was determined in lysates of embryos injected with 20 pg of pSiaLuc and indicated mRNAs. Results shown in relative light units represent means of three samples±s.d., with five embryos in each sample.