Fig. 2. Expression of Gdf11 in the neural tube results in rostral displacement of motor neuron column and pool positions. (A) Raldh2 and (B) Pea3 expression in the spinal cord and (C,D) neurofilament (3A10) staining in HH stage 29 embryos 5 days after Gdf11electroporation. The electroporated side (EXP) is on the right of all panels, while the control side (CTL) is on the left. Rostral is towards the top and caudal is towards the bottom. White brackets indicate the origins of the spinal nerves that innervate the forelimbs in C and the hindlimbs in D. Green asterisks mark the first (C) and the last (D) spinal nerves that innervate the thoracic levels. Broken black lines mark the rostral boundaries of the hindlimbs in D. Scale bars: 0.5 mm.