Fig. 5. XHRT1, but not esr9, esr10 or Xhairy2b inhibits pronephric distal tubule and duct formation. (A-R) Whole-mount in situ hybridization of embryos injected with 500 pg of mRNA encoding the indicated inducible constructs together with ß-galactosidase mRNA analysed at stage 16 for N-tubulin (+Dex, stage 12; A,D,G,J,M,P) or stage 28 for Evi1 expression (+Dex, stage 18; B,C,E,F,H,I,K,L,N,O,Q,R). Embryos at neurula stage are viewed from the dorsal side, injected side downwards. Lateral view of control and injected sides of stage 28 embryos are shown. (A-C) Embryos injected with hGR/Su(H)/Ank mRNA showed an inhibition of N-tubulin and Evi1 expression (C, arrow). (D-R) Although overexpression of all bHLH-O genes inhibited N-tubulin in the neural plate, only XHRT1 overexpression inhibited Evi1 expression (F, arrow) in the pronephros. Lines in A,D,G,J,M,P indicate the injected and uninjected sides.