Fig. 6. Comparison of the ability of hGR/Su(H)/Ank and XHRT1-MT-hGR to affect the expression of proximal tubule and glomus markers. (A) Activation of Notch signaling until stage 25 expands xWT1 efficiently, while later activation had no effect. XSMP-30 expression was increased in some embryos activated between stages 22 and 27. Embryos were co-injected with hGR/Su(H)/Ank and ß-galactosidase mRNA. Injected embryos were treated, or not, with Dex at the indicated times and analysed at stage 32-35. Changes in the expression of xWT1 and XSMP-30 at each stage were scored in individual embryos by comparing the injected and injected sides in at least two different injections. Embryos were classified into three phenotypes (no changes, increase or decrease). n, number of cases analysed. (B-M) Control and injected sides of embryos injected with the indicated mRNA together with ß-galactosidase mRNA, treated with Dex at stage 18 (xWT1, nephrin) or 25 (XSMP-30) and analysed with the indicated probes. Note that hGR/Su(H)/Ank expands xWT1, nephrin and, in a few cases, XSMP-30, while XHRT1-MT-hGR has no effect.