Fig. 9. XHRT1 specific function in the pronephros is dependent on its C-terminal region. (A) Schematic representation of the XHRT1, XHes2 and chimeric proteins. All constructs encode Myc tag and hGR fusion proteins (not represented). The numbers correspond to the amino acids of the protein domains. (B) Western blot analysis of the expression level of XHRT1, XHes2 and chimeric proteins. Extracts prepared from animal caps derived from embryos injected with 250 pg of each construct were immunoblotted with anti-Myc and anti-ß-tubulin antibodies. (C) Comparison of the activity of XHRT1, XHes2 and chimeric proteins. Embryos were injected with 500 pg mRNA of each construct. The embryos were treated with dexamethasone at stage 18 and fixed at stage 26. Changes in Evi1 expression were scored as in Fig. 6A.