Fig. 3. ett and arf4 affect leaf shape and trichome
distribution. (A) Successive rosette and inflorescence leaves from
wild-type and mutant plants, arranged from the base (left) to the tip (right)
of the stem. ett and arf4 suppress the narrow, elongated
phenotype of the first two zip leaves. Later leaves of double mutants
have rounder tips than zip-2, and the distal portion of the leaf
blade is often wider than the proximal portion. ett and arf4
do not affect the serration of the leaf blade. (B) The L:W ratio of the
leaf blade of successive leaves of wild-type, ett-7 and
arf4-2 plants (n=10 plants of each genotype;
±s.e.m.). In wild-type plants, this ratio increases gradually until
leaf 8, after which it remains constant until flowering. ett-7 and
arf4-2 cause leaves 4-7 to be slightly rounder than normal.
(C) ett-15, ett-7 and arf4-2 increase the number of
leaves without abaxial trichomes (black) in both a wild-type and a
zip background. This is associated with a compensatory decrease in
the number of adult leaves (grey; n
18, ±s.e.m.). The
number of cauline leaves is indicated by the white bar. The numbers above each
bar represent the percentage of flowers with a split septum, based on an
analysis of the first 10 flowers of five plants of each genotype.
ett-15 and arf4 partially suppress the septum splitting
observed in zip. Other arf4 mutations also delay abaxial
trichome production in a zip-2 background. (D) The number of
leaves without abaxial trichomes among plants segregating ett-7 and
zip-2. ett-7 has a semi-dominant effect on abaxial trichome
production.