Fig. 6. Odd1 can induce early nephric markers when ectopically expressed in the somites. Control chicken embryos (A,D) and embryos electroporated with Mes-Odd1 expression vector (B,C,E) were stained with immunofluorescence against Pax2 (A,B) or GFP (C), or by whole-mount in situ hybridization for Lim1 (D,E). Ectopic cells expressing Pax2 or Lim1 are found in the somite region (arrowheads in B,E). In B and C, note that GFP cells in the lateral plate (arrows) do not express ectopic Pax2. im, intermediate mesoderm; nt, neural tube; s, somite.