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Figure 1


Fig. 1. Embryonic lethality of Sall4-deficient mice. (A) Targeting strategy of Sall4. Ovals represent the zinc-finger domains. E, EcoRI. (B) Southern blot analysis using the probes described in Fig. 1A. (C) Hematoxylin and Eosin staining of wild-type (+/+) and Sall4-deficient (-/-) embryos at E6.5. Arrow indicates epiblast. (D) In situ hybridization of Sall4, epiblast markers (Fgf4, Nodal and Oct3/4), and a marker for trophectoderm and extra-embryonic ectoderm (H19) in wild-type (+/+) and Sall4-deficient (-/-) embryos at E5.8. H19 signal is absent in the epiblast of both +/+ and -/- embryos. Black arrowhead, epiblast; white arrowhead, extra-embryonic ectoderm. Serial sections from two wild-type and two Sall4-deficient embryos are shown. Left three columns and right two comulns are from different embryos.