Fig. 6. Mislocalization of Sall4 from the heterochromatin by a truncated Sall1 that functions in a dominant-negative manner. (A) Requirement of the C-terminal zinc finger (Zn4) of Sall4 for heterochromatin localization (rectangle). Zn4 is also sufficient for heterochromatin localization (broken outline). Mutants of Sall4-GFP fusion were expressed in NIH 3T3 cells. Zinc-finger clusters are categorized as Zn1, Zn2, Zn3 and Zn4, as shown. (B) Requirement of the C-terminal zinc fingers (Zn4 and Zn5) of Sall1 for heterochromatin localization (rectangle). Zn4 and Zn5 are also sufficient for heterochromatin localization (broken outline). Mutations in Zn2 and Zn5 also show a defect in heterochromatin localization, though these two clusters are not sufficient for proper localization in the heterochromatin. (C) Co-transfection of truncated Sall1 (Sall1^1-435-DsRed) and Sall4-GFP into NIH 3T3 cells. Heterochromatin localization of Sall4 is disrupted by C-terminally truncated Sall1.