Fig. 3. MTS24 co-staining with described markers for epidermal stem cells. (A-C) Frozen section of dorsal skin showing expression of keratin 14-Cy3 (A; red) and MTS24-FITC (B; green) and merged (C) in hair follicle. Arrowhead highlights co-localisation of keratin 14 expression and MTS24 labelling. (D-F) Expression of CD34-Cy3 (D; red) and MTS24-FITC (E; green) and merged (F) in dorsal skin. Note that CD34 expression (arrowhead) was found in a different location within the hair follicle than MTS24-staining (asterisk). (G-I) Wholemount of tail epidermis showing no co-localisation between keratin 15-FITC (G; green) and MTS24-Cy3 (H; red) labelling within the hair follicle. Merged image is shown in (I). (J) Labelling with MTS24-Cy3 (red) and FITC anti-mouse (green) secondary antibody alone, shows that staining of sebaceous gland is non-specific in (G,I). (K) Wholemount of tail epidermis showing 6 integrin-FITC (green) and MTS24-Cy3 (red) co-staining (arrowhead). SKH-1 (L,M) or wild-type (N,O) neonatal mice received repeated injections with BrdU to generate label-retaining cells. Frozen sections (L-N) or tail wholemounts (O) were collected at 1 day (L), 6 weeks (M) or 10 weeks (N,O) after the last injection with BrdU. Tissue was labelled for BrdU-FITC (green) and MTS24-Cy3 (red). Nuclear counterstain was DAPI (L,M,N; blue). Arrowheads indicate BrdU label-retaining cells (L-O). In each panel the bulge area is bracketed. SG, sebaceous gland; HF, hair follicle; IFE, interfollicular epidermis. Scale bars: 50 µm.