Fig. 3. DFer colocalises with DE-Cadherin and is apical to Fasciclin 3. (A) At stage 12, DFer protein localises to cell-cell junctions (arrow) in leading edge cells and the dorsal epidermis. (B) DFer is lost from the border with the amnioserosa by stage 14 (arrow). (C-E) DFer colocalises with DE-Cadherin in the dorsal epidermis at stage 14, but is largely absent from the adherens junctions in the amnioserosa. (F-H) At stage 13, DFer overlaps extensively with DE-Cadherin. (I,J) At stage 14, the cell-cell junction staining of (I) DFer (red, arrow) is apical to (J) Fas3 (green, arrow). Confocal sections in I and J are 1 µm apart.