Fig. 9. ß-catenin levels and phosphorylation state are altered in dfer mutants. (A,B) ß-catenin immunoprecipitated from (A) dfer¹ and (B) dfer^gof late stage embryo extracts, probed with anti-arm (ß-catenin) and anti phosphotyrosine. ß-catenin is hypophosphorylated in dfer¹ embryos (21% relative to yw control embryos) and hyperphosphorylated in dfer^gof embryos (168% relative to yw). (C) Levels of ß-catenin are reduced in the epidermis of dfer^gof embryos. DE-Cadherin may also be reduced to a lesser degree. (D) Western blots on total embryo extracts confirm that ß-catenin is depleted in dfer^gof mutants. (E) Loading control (anti-actin).