Fig. 6. Behavior of WAVE complex components in Nap1^khlo mutants. (A) Western blot analysis of WAVE1 protein in E8.5 embryos, normalized to -tubulin levels. (B-E) Immunofluorescent staining shows that Sra1 (B,C) and Abi1 (D,E) are localized to the surface of wild-type (B,D) but not Nap1^khlo (C,E) primary mesodermal cells. Scale bars in B-E: 25 µm. (F-I) Actin structures in wild-type (F,H) and Nap1^khlo (G,I) epithelial (F,G) and mesodermal (H,I) cells, visualized by phalloidin staining. (J,K) Localization of the actin-binding protein cortactin (green) and DAPI (blue) in wild-type (J) and Nap1^khlo (K) mutant mesodermal cells. Scale bars in F-K: 50 µm.