Fig. 7. Cell migration defects in Nap1^khlo explants and embryos. (A,B) Wild-type (A) and Nap1^khlo (B) primitive streak explants stained with DAPI (blue) and antibodies against E-cadherin (red) to distinguish the E-cadherin-expressing epithelial cells from E-cadherin-negative mesodermal cells. (C-F) Transverse sections of wild-type (C,E) and Nap1^khlo (D,F) E7.5 embryos stained for expression of E-cadherin. Brackets mark the layer of mesenchymal cells adjacent to the primitive streak; whereas a single layer of mesoderm cells lie adjacent to the primitive streak in wild type, 3-4 layers of mesoderm cells underlie the Nap1^khlo primitive streak. Scale bars: 100 µm.