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Figure 6


Fig. 6. tbx-35 is required to specify ectopic MS-like cells. (A) Ectopic MS-type muscle (hlh-1::GFP) in tbx-35(+); pie-1(RNAi); pal-1(RNAi) embryos. (B) Ectopic pharynx cells (ceh-22::GFP, pseudocolored yellow) in a tbx-35(+); mex-1(RNAi); pie-1(RNAi) embryo. (C) Very few hypodermal cells (lin-26::GFP, pseudocolored cyan) are made in a tbx-35(+); mex-1(RNAi); pie-1(RNAi) embryo (an average of 6.5±2.1 cells, n=16). (D) Dramatic reduction in number of muscle cells in a tbx-35(-); pie-1(RNAi); pal-1(RNAi) embryo. (E) Near absence of pharynx in a tbx-35(-); mex-1(RNAi); pie-1(RNAi) embryo. (F) Production of hypodermal cells in a tbx-35(-); mex-1(RNAi); pie-1(RNAi) embryo (an average of 26.7±3.9 nuclei, n=11). Simultaneous depletion of pal-1 greatly reduces the production of these extra hypodermal cells (Table 1).