Fig. 8. Branch integrity phenotypes of cell adhesion downregulation. (A,B) Posterior tracheal metameres of shg² (A) and cv-c⁷ (B) stage 15 or 16 embryos focused on the DT and DBs. Note the presence of branch interruptions and branches with cells only connected by cytoplasmic extensions (arrows). (C) Single confocal section of a stage 16 embryo labelled with anti-actin (red in C; greyscale in C') and anti-Trh (green). In shg mutants, cortical actin is greatly reduced compared with the wild type (Fig. 5F). (D) Projection of confocal sections of a stage 16 embryo labelled with anti-DCAD2 (red in D; greyscale in D') and anti-Trh (green). Note that in cv-c mutants the levels of DCAD2 are lower than in the wild type (see Fig. 5A), and that staining in the DBs is less conspicuous (arrow).