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Figure 5


Fig. 5. Infection with RCAS:CA-ß-catenin induces ectopic peripheral markers. (A-D) ISH for Collagen IX (A,B) and Bmp7 (C,D) at E5.5 and E6.5, respectively. An uninfected retina exhibits marker gene expression in the ciliary and iris epithelia (A,C), but not in the central retina (B,D). Arrow in D indicates optic nerve exit. (E-H) ISH for Collagen IX (E) and Bmp7 (G) in E5.5 retinas infected with RCAS:CA-ß-catenin. IHC for {alpha}-gag marks the area of viral infection (red, F,H). Note that the strong AP signal quenches the fluorescence signal in some areas. (I-L) ISH for WFDC1 (I), Wnt2b (K) and Lef1 (L) in E7.5 retinas infected with RCAS:CA-ß-catenin. Note that some of the thin and/or folded regions show ectopic peripheral marker induction in I,K,L (arrows). IHC for {alpha}-gag marks the area of viral infection (red, J). (M,N) IHC of {alpha}-SMA at E7.5 in an RCAS:CA-ß-catenin-infected retina shows ectopic induction of SMA (red) in the abnormally thin and folded region (arrow). Arrowheads indicate the lack of SMA induction in the central retinal area. Viral infection was visualized with {alpha}-gag antibody (green, N).