Fig. 2. Lhx5 partially rescues forebrain deficiencies induced by ectopic Wnt signaling. (A) lhx5 gain of function partially rescues the eyeless phenotype of wnt8a mRNA-injected embryos. Injected embryos were scored after the Prim-5 stage (24 hours post-fertilization). The percentages of embryos eyeless (blue) or with two eyes (green), or malformed as a result of dorsalization or injection (yellow), were measured. (B-D) lhx5 gain of function partially rescues eye development in mbl (axin1) mutants. Ventral views, rostral to the left. (E-G) lhx5 gain of function partially restores emx3 expression in mbl (axin1) mutants. Genotypes of mutant and partially rescued embryos were verified by RFLP. (H-J) lhx5 gain of function partially restores six3b expression in mbl (axin1) mutants. WT, wild-type embryos. Scale bar in J: 150 µm for B-D; 250 µm for E-J.