Development -- Laubinger et al. 133 (16): 3213 Figure IG7

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Fig. 7. CO protein levels are strongly increased in spa1 spa3 spa4 triple mutants. (A) RT-PCR analysis of CO and UBQ10 transcript levels in wild-type plants, spa1 spa3 spa4 mutants and plants of a transgenic 35S-CO overexpressing line. (B) CO protein levels in nuclear extracts of wild-type plants, spa1 spa3 spa4 mutants and plants of a transgenic 35S-CO overexpressing line. As a loading control, blots were reprobed with an antibody against histone H3A. Plants were grown in LD for 12 days and harvested at ZT16.