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Figure 1


Fig. 1. Col2{alpha}1-cre3 removal of ß-catenin function results in a failure to develop terminally differentiated osteoblasts. Histological analysis and in situ hybridization of 35S-labeled riboprobes to sections of E18.5 tibia from (A-I) ß-cateninc/c (wild type) and (J-R) Col2{alpha}1-cre3;ß-cateninc/c (mutant) embryos, with specific anti-sense riboprobes for marker genes that identify states of osteoblast and chondrocyte differentiation. Osteoblast: (D,M) Col1{alpha}1, (E,N) Runx2, (F,O) osterix1 and (G,P) osteocalcin. Chondrocyte: (H,Q) Col2{alpha}1 and (I,R) Col10{alpha}1. Boxed areas in B and K are presented at higher magnification in C and L, respectively; black arrows indicate the absence of ossification in the mutant periosteum. White arrowheads indicate the invading wedge of mesenchymal cells into the marrow cavity; black arrowheads indicate sites of ectopic expression of chondrocyte marker genes.