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Figure 5


Fig. 5. Osx1-GFP::Cre removal of Smo function does not affect osteoblast differentiation. Skeletal preparations of (A,B) Smoc/n (wild type) and (F,G) Osx1-GFP::Cre;Smoc/n (mutant) fore- and hindlimbs. Histological analysis (C,D,H,I) and in situ hybridization with specific anti-sense 35S-labeled riboprobes for the terminal osteoblast differentiation marker osteocalcin (E,J) to sections of E18.5 tibia from wild-type (C-E) and mutant (H-J) embryos.