Fig. 1. Basement membrane disruption and radial glial endfeet detachment in laminin 1III4^-/-, perlecan^-/- and 6-integrin^-/- cortex at midneurogenesis. Fluorescent micrographs of immunostained (as indicated in the panels) frontal sections of telencephali of embryonic day (E) 14 wild-type and mutant littermates. (A-D) Laminin immunoreactivity underneath the pial surface (arrowheads in A) and surrounding the blood vessels (arrows in A). Insets (a-d) depict representative high-power views of the BM in the respective mouse line. Arrowheads in C,D indicate disruptions of the BM. (E-J) The absence of BLBP-immunoreactive radial glia endfeet (arrowhead in E,G) in the laminin 1III4^-/- (F,H), perlecan^-/- cortex (I) and 6 integrin^-/- cortex (CTX; J) compared with wild type (E,G). Ectopic BLBP-positive cell somata in the laminin 1III4^-/- cortical plate (CP) (arrows in F,H). The broken white line (E) indicates the ventricular surface (VS). CTX, cerebral cortex; GE, ganglionic eminence; VZ, ventricular zones. Scale bars: 200 µm in A-D; 50 µm in E,F; 25 µm in G,H; 100 µm in I,J.