Development -- Haubst et al. 133 (16): 3245 Figure IG4

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Fig. 4. Neuronal subtypes in the E18 laminin ${gamma}$ 1III4^-/- cortex. Micrographs of in situ hybridization for the layer-specific mRNAs of Cux2, Rorb (RORß) and Er81 (A-F), and immunostaining for interneurons (G-J) in E18 wild-type and laminin ${gamma}$ 1III4^-/- cortex. Cux2- and Rorb-expressing layer II-IV neurons are misplaced to the position of Er81-positive deep layer neurons in the laminin ${gamma}$ 1III4^-/- cortex (E,F). The red arrows indicate the layer specific gene expression. Conversely, GABAergic interneurons containing calretinin (red in G,H) or calbindin (red in I,J) were detected in the outer part of the laminin ${gamma}$ 1III4^-/- cortex (H,J) in contrast to their scattered position in the wild-type cortex (G,I). Early born reelin-immunoreactive cells (green in G-J) are mostly calretinin positive (G',H') and are not increased in the laminin ${gamma}$ 1III4^-/- cortex (H) compared with wild type (G). Scale bars: 200 µm in A-F; 50 µm in G-J.