Fig. 6. Ed is required for dorsal closure. (A,A') Stage 14 embryo, dorsal view, zippering phase of dorsal closure (Jacinto et al., 2002). (A) Arm immunoreactivity highlights the amnioserosa (arrow) and surrounding lateral epidermis. (A') Ed immunoreactivity is absent from the amnioserosa (arrow). (B,C) Arm staining of ed^MZ mutant embryos at the zippering phase (B) and termination phase (C) of dorsal closure. There are gaps along the dorsal midline (arrow) and misaligned segments (arrowheads). (D-D'') Successive images of a live wild-type embryo expressing GFP-moesin during zippering (D,D') and termination (D''). Accumulation of GFP-moesin at the leading edge highlights the contractile cable (D,D', arrows). (E-E'') Live imaging of dorsal closure in an ed^MZ embryo expressing GFP-moesin. GFP-moesin does not accumulate at the leading edge (E,E', arrows), and the gaps and segments are misaligned in the termination phase (E'').