Fig. 2. Loss of sax function expands pMad distribution in wing discs. Intensity plots (A,B,C,D) of the relative fluorescence (y-axis) of (A',B',C',D') pMad (red) across equivalent regions of the wing pouch (broad white line). (A) Wild type, (B) sax⁴/Df(2R)H23, (C) Df(2R)H23/+ and (D) gbb¹/+. (A'',B'',C'',D'') brk expression (green) (brk^X47-LacZ). Thin white lines mark the extent of brk repression from the center of wing pouch. All images were captured using the same microscope settings.