Fig. 4. Ice is required for normally occurring cell death in the adult wing and pupal retina. (A) en::GFP expression in the wing of a wild-type adult (eclosed for less than 1 hour). (B) en::GFP expression in the wing of a wild-type adult (eclosed for 2 hours). (C) en::GFP expression in the wing of a Ice¹ adult (eclosed for 24 hours). (D,E) Forty-hour pupal eyes stained with anti-Dlg. (D) Wild type; (E) Ice¹. The area in which the number of interommatidial cells was counted for each genotype is indicated in D by the broken line. Several regions with extra cells are indicated with arrows in the mutant (E). Animals homozygous for Ice¹ lack zygotic but not maternal Ice.