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Figure 9


Fig. 9. Antibodies that recognize cleaved versions of Ice are accurate reporters of Ice activation in irradiated wing discs, but not in embryos and spermatids. (A-R) X-irradiated wing discs of various genotypes stained for TUNEL (A,C,D,F,G,I,J,L,M,O,P,R), anti-active Ice (B,C,H,I,N,O) or CM1 (E,F,K,L,Q,R). Genotypes are as indicated in the figure. Wild-type (S,U) or Ice{Delta}1 (T,V) embryos stained with anti-active Ice (S,T) or CM1 (U,V). (W-Z) Wild-type (W,Y) or Ice{Delta}1 testis stained with anti-active Ice (W,X) or CM1 (Y,Z).