Fig. 3. Rescue, expression, site of action and domain requirements of fozi-1. (A) Rescue of the fozi-1 mutant phenotype. The constructs do not contain the first, non-coding exon of the fozi-1 gene, which is located >7 kb upstream of the ATG-containing second exon (Fig. 2A). The right column indicates rescue of the fozi-1 defect, i.e. suppression of aberrant lim-6 expression in ASER, and the left column indicates suppression of normal lim-6 expression in ASEL fate, caused by ectopic expression of fozi-1. All constructs show similar expression levels and exclusive localization to the nucleus. (B) A representative fozi-1::gfp-expressing animal, showing gfp expression in ASER but not ASEL, and in the two olfactory neurons AWCL and AWCR. Broken lines approximately indicate the head of the worm. The insets show that fozi-1::gfp predominantly localizes to the nucleus. The red `cyto' marker is dsRed2 protein, expressed under control of the ceh-36 promoter (otIs151 transgene). (C) Three out of four fozi-1::gfp-expressing transgenic, wild-type lines display varying levels of asymmetric gfp expression in ASER. Circles indicate absent, ASEL alone, ASEL and ASER, and ASER alone, respectively.