Fig. 2. Axon maintenance defects in sax-8 mutants. (A) Axonal maintenance defect of the two PVQ neurons in sax-8(ky188) mutants. Arrow indicates region of axon flip-over. (B) Quantification of PVQ flip-over defect. (L1: n=40-102; A; n=174-399). `L1' are freshly hatched first larval stage animals. `A' are young adults that have just molted. Error bars indicate standard error of proportion. (C) PVQ flip-over defect of ky188 mutants can be suppressed by paralysis, induced pharmacologically (50 µM levamisol), or genetically, using mutants animals that are paralyzed because of ultrastructural muscle defects [unc-54(e1092) and unc-97(su110)] or neuronal signaling defects [unc-36(e251) and unc-13(e1091)]. The ky188 data are the same as in B. Adult animals were scored (n=122-206). (D,E) Axonal maintenance defects of AVK and RMEV neurons. Animals were scored as young adults only, as these reporters do not allow the visualization these axons in early larvae (n=69-244 for untreated animals). The axonal defects are profoundly suppressed by paralysis using levamisol at 50 µM (n=131, 89, respectively; E). Error bars indicate standard error of proportion.