Fig. 6. Positional cloning of sax-8. (A) Summary of sax-8 genetic map data. Based on the observed recombination frequencies (as shown), we calculated the position of sax-8 to be at -0.95±0.45. (B) Gene structure of sax-8/dig-1/K07E12.1, taken from www.wormbase.org. The sax-8/dig-1 defects can be rescued with cosmids K07E12 and R05H11 (see Table 1) and with cosmid K07E12 alone (data not shown). (C) Domain structure of DIG-1 and mutant alleles.