Fig. 2. In situ hybridization analysis shows epithelial-specific expression of the Cre transgene in the developing palate of [K14-Cre; R26R] embryos. (A) Diagram showing mouse secondary palates at E14.5 and the positions of sections shown in C,D,E. (B) In situ hybridization showing high expression of the Cre transgene in the entire palatal epithelium (arrowheads) at E13.5. (C,E) In situ hybridization showing that the Cre transgene expression domain covers almost the entire palatal epithelium in the anterior (C) and posterior (E) regions of the palate at E14.5. (D) In situ hybridization showing that the Cre transgene is expressed only in the oral side of palatal epithelium (arrowheads) and the triangular region (arrow) in the middle region of the palate at E14.5. Scale bars: 50 µm.