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Figure 5


Fig. 5. Hematoxylin and eosin staining and TUNEL assay reveal abnormal triangular areas and a lack of apoptosis in the Apaf1 mutant palate. (A) An E15.5 wild-type palate showing a normal triangular area (arrow). (B) An E15.5 Apaf1 mutant palate containing an enlarged and highly compacted triangular area (arrow). (C) Apoptotic cells were visualized by TUNEL assay in the triangular area of the wild-type palate at E15.5 (arrow). (D) No TUNEL-positive cells were detected in the triangular area of the Apaf1 mutant palate at E15.5 (arrow).