Fig. 1. cpa or cpb mutant clones are extruded from the wing blade epithelium. All panels show third instar wing discs. (A-C) Standard confocal sections. (A) cpa^107E, (B) tsr^110M and (C) capt^E636 mutant clones are marked by the absence of GFP (green). (D-H) Optical cross-sections through the wing disc epithelium. Mutant clones are positively labeled with GFP (green) and sections are stained with anti-Dlg (red) and anti-Arm (blue) to outline apical cell membranes. (D) cpa^107E; (E) cpb^M143; (F) tsr^110M; (G) capt^E593; (H) cpa^107E mutant clones overexpressing full-length cpa. cpa or cpb mutant clones are extruded basally in the wing blade primordium (red arrows), but not the notum; this extrusion is rescued by full-length cpa. tsr and capt mutant clones are not extruded. The white arrows define the wing blade region. Dorsal is towards the left on optical cross-sections in this and subsequent figures.