Fig. 6. The N terminus of Slug binds to Ppa and can transfer this interaction to Sox10. (A) Embryos were co-injected with Ppa and wild-type Slug, Slug-N or Slug-C. Following immunoprecipitations using an -Myc antibody (Slug/mutants), bound Ppa (arrow) was detected by -Flag immunoblot. Ppa binds the N terminus of Slug, but not the C terminus. (B) Embryos co-injected with Ppa and Slug, Sox10 or a Slug N-Sox10 fusion construct were collected for IP/western blotting. The addition of Slug M1-H64 is sufficient to transfer Ppa binding to Sox10 (arrow). (C) Serial Slug deletion mutants were co-injected with Ppa to map the Ppa binding region. (D) Co-immunoprecipitation experiments showing that a Slug mutant with deletion of amino acids P31 to H64 no longer interacts with Ppa.