Fig. 4. Chimeric analysis of Apc^Min/Min mutants. (A) T (red) and EGFP (green) in situ hybridization on chimeras with low epiblast contribution from wild-type YFP-labeled ES cells in Apc^Min/Min mutant embryos at 5.5 dpc. (B-D) Chimeras with high epiblast contributions from wild-type ES cells in wild-type and Apc^Min/Min mutant embryos. Chimeras (5.5-6.0 dpc) were hybridized with probes against EGFP (B,C, green) and (in red) Hex/Bmp4 (marks the ExE; B), Gsc or Cer1 alone (C,D). The arrowheads indicate boundaries of the DVE/AVE.