Fig. 5. Asymmetric expression of molecular markers. (A,B) Immunohistochemistry of ß-catenin (red) and E-cadherin (blue, B only) on 4.5 dpc embryos. B is a magnified right lateral view of A. The arrowheads indicate the trophoblast expressing ß-catenin throughout the cell. (C) In situ hybridization of Hex and Pem at 4.5 dpc. Arrowheads indicate the enhanced expression of Hex and Pem. (D)In situ hybridization of Hex (red) and Oct4 (blue) at 4.75 dpc. (E-H) Embryo (4.5 dpc) labeled with ß-catenin antibody (green), with propidium iodide for nuclei (red) and with Hex probe (blue). E and F are at the same z section, whereas H is in a different plane of the same embryo. G is an overlay of E,F,H. The arrowheads indicate the same trophoblast cell bearing nuclear ß-catenin. The arrow in G,H indicates the enhanced expression of Hex. (I,J) In situ hybridization of Lefty1 (red) and immunolocalisation of Dab-2 (green) at 4.5 dpc. (K) In situ hybridization of Lefty1 (red) and immunolocalisation of Dab-2 (green) at 4.75-5.0 dpc. The arrowhead indicates the center of Dab-2-expressing cells. Ep, epiblast; Tr, trophectoderm.