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Figure 8


Fig. 8. Normal activation of the ERK/MAPK cascade in rescued Map2k1{Delta}/{Delta} embryos. (A) Placental phenotype of Map2k1{Delta}/{Delta} embryos. Gross morphology of Map2k1{Delta}/{Delta} embryos revealed hemorrhages (white arrowhead) characteristic of the Map2k1-/- null phenotype. Comparative Hematoxylin-Eosin staining of E10.5 wild-type and Map2k1{Delta}/{Delta} placenta sections showed the absence of maternal sinuses and fetal blood vessels intermingling in mutant specimens. The black arrows and arrowheads indicate the fetal blood vessels and the maternal sinuses in the labyrinthine region, respectively. (B) ERK/MAPK cascade activation in Sox2Cre;Map2k1{Delta}/{Delta} embryos having a normal development of their extra-embryonic structures. E10.5 embryos produced from mating between Sox2Cre;Map2k1+/{Delta} males with Map2k1+/floxed females were used for protein extracts. Western blots were probed with antibodies against MAPK1, MAP2K1, MAP2K2, phospho-MAPK1/MAPK3 and phospho-MAP2K1/MAP2K2.