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Figure 4


Fig. 4. UNC-5::GFP expression in non-neural cells and UNC-40::GFP expression are normal in unc-51 and unc-14 mutants. (A-J) Wild type (A,D,H); unc-51(e369) (B,E,I); unc-14(e57) (C,F,J); unc-40(n324) (G). (A-C) Dotted lines show the approximate outline of a distal tip cell (DTC). Anterior is left. Dorsal is up. (D-F) Excretory cells. Anterior is upper left. Dorsal is upper right. (G) Morphology of DD/VD motoneurons of unc-40(n324) mutants. Anterior is upper left. Dorsal is upper right. Arrows show abnormally positioned axons. (H-J) UNC-40::GFP expression. UNC-40::GFP was expressed at the cell surface of DD/VD cells (H) and its localization was normal in unc-51 (I) and unc-14 mutants (J). Scale bars: 10 µm in A-F,H-J; 100 µm in G.