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Figure 1


Fig. 1. Dye coupling of PGC cells with their neighbours. (A,B) Representative images obtained following intracellular microinjection of fluorescent dye sulforhodamine 101 into PGCs contained in hindgut explants from both Cx43{alpha}1 wild-type (+/+) and homozygous Cx43{alpha}1KO (-/-) embryos (E9.5). Both A and B comprise three images superimposed: a DIC image, green Oct4-GFP fluorescence image (PGC), and a red sulforhodamine 101 fluorescence image. (C) Dye spread was quantified by calculating total area of dye spread after 5 minutes (2 minutes' active microinjection plus 3 minutes' passive spread). PGCs in Cx43{alpha}1KO (-/-) explants displayed a significant reduction in their ability to pass dye into their neighbors. For this analysis, 8, 4 and 7 microelectrode impalements were carried out, respectively in four Cx43{alpha}1 +/+, four Cx43{alpha}1 +/- and five Cx43{alpha}1 -/- embryo explants.