Fig. 2. PUF-8::GFP and FBF-2::GFP expression during vulval development. (A) Structure of the translational puf-8::gfp and fbf-2::gfp reporters. (B,D,F,H) Time-course analysis of PUF-8::GFP expression in the vulval cells from the L2 until the L4 stage with (C,E,G,J) the corresponding Nomarski images. For a semi-quantitative analysis of the expression patterns, see Fig. S1 in the supplementary material. (K,L) PUF-8::GFP expression in gonad-ablated eff-1(hy21) animals, and the corresponding Nomarski image. Note that despite the extra round of cell divisions in P4.p and P5.p descendants of gonad-ablated eff-1 mutants no vulval differentiation was observed. (M-R) FBF-2::GFP expression, and the corresponding Nomarski images, from the early L3 until the L4 stage. In all panels, anterior is to the left and ventral is to the bottom. Scale bars: in C,L,N and in the inset of J, 10 µm.