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Figure 3


Fig. 3. fbf-1 and fbf-2 inhibit 1° cell fate specification. Analysis of EGL-17::YFP expression in mid-L3 larvae at the Pn.px or Pn.pxx stage (left side) and in L4 larvae at the Pn.pxxx stage (right side). (A-D) Wild-type, (E-H) gap-1(ga133), (J-M) gap-1(ga133); puf-8 RNAi and (N-Q) fbf-1(ok91) fbf-2(q704); gap-1(ga133) larvae. In all panels, anterior is to the left and ventral is to the bottom. In the graphs, white indicates no EGL-17::YFP expression, grey low expression and black high expression. The arrows in L and P indicate ectopic induction of distal vulval cells; the arrowhead in P indicates an example with expanded EGL-17::YFP expression in VulA and VulB, and the resulting defect in the 2° fate execution. Scale bars: in A,C, 10 µm.