Fig. 3. CDC-42 and RHO-1 are required for PAR-6 localization. (A) Embryos expressing GFP-PAR-2 were stained for GFP, PAR-6, microtubules (MT, green) and DNA (blue). In control embryos, PAR-6 localizes to the anterior pole, whereas PAR-2 localizes to the posterior pole. In cdc-42(RNAi) embryos, PAR-6 is not detectable at the cortex and PAR-2 is found at the entire cortex. In cdc-42(RNAi);par-2(RNAi) embryos, PAR-6 relocalizes anteriorly, but only at reduced levels. (B) Time-lapse images of GFP-PAR-6 polarity establishment in control and rho-1(RNAi) embryos. Times (seconds) are relative to nuclear envelope breakdown.