Fig. 1. Fkh regulates expression of SG1 and SG2. (A,B) Expression of SG1 and SG2 RNA and (D) SG1 protein was absent in fkh mutants. (C) Expression of pipe was unaffected by loss of fkh. (E) SG2 (red) colocalized with the ER marker KDEL (green) and SG1 (green) colocalized with SG2 (red) (top and bottom panels, respectively). We examined expression of genes with a fkh null mutation in the background of the H99 deficiency, which removes the pro-apoptotic genes; this background allows salivary gland cell survival in a fkh mutant (Myat and Andrew, 2000). Expression of pipe, SG1 and SG2 were the same in fkh mutants that also did not carry the H99 deficiency.