Fig. 2. Expression patterns of Escargot, Worniu and Wingless in the peripheral eye. (A-F) All micrographs are flat mounted pupal retinas of 32 hours APF. (A-A'') Pupal eye stained for Escargot (red) and Elav (green). Escargot is expressed in the HC, PR and peripheral 2°/3° pigment cells. (B-B'') Cir1-Gal4>UAS-lacZ eyes stained for lacZ (red) and Elav (green). Cir1 and Escargot show the same expression profile. (C-C'') Escargot (red) and Cut (green, specifically labels cone cells) are co-expressed in the marginal cone cells. (D-D'') Escargot (red) and Homothorax (green) co-expressed in the head capsule (arrow). Homothorax is also expressed in the central photoreceptors of the outer ommatidia (those that die) and an inner ring that will eventually form the DRO. (E-E'') wg-lacZ eyes stained for lacZ (green), Cut (red) and Elav (blue) showing Wg expression in the perimeter cone cells. (F-F'') wg-lacZ eyes stained for lacZ (green), Wg (red) and Elav (blue) showing the absence of both Wg expression in the perimeter photoreceptor cells. (G) Summary of the expression patterns of Esg and Wg. Top panel: a key to the cell types depicted below. Bottom panels: Schematic summary of Wg and Escargot expression in the eye periphery at 32 hours APF. Escargot is expressed in the HC, peripheral 2°/3° pigment and cone cells. Wg is expressed in the HC and peripheral cone cells.