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Figure 3


Fig. 3. Wg signaling regulates the expression of Snail family factors. (A) A clone of actin-wg in adult RR eye induces ectopic expression of the transcriptional reporter (arrow). (B-B") A clone of actin-wg (red) in Cir1 pupal eye (~32 hours APF) showing ectopic expression of the transcriptional reporter (green). (C-C") An actin>Gal4; UAS-{Delta}Arm clone in pupal retina (~32 hours APF) marked by GFP (green) shows ectopic Escargot (red) non-autonomously. (D-D") A large dsh[V26] clone in the pupal eye periphery (~32 hours APF) marked black by the absence of GFP. Peripheral Esg (red) is almost entirely abolished. The faint lattice of staining is the incipient Esg expression in the main retina 2°/3° pigment cells - this is not Wg dependent. (E-E") An arr[2] clone in the pupal eye edge (~32 hours APF) marked black by the absence of GFP. Escargot expression (red) is lost in the clone. (E'') A merge of the two with an additional stain for Cut (blue). The arrowhead indicates a cone cell that would normally express Escargot but fails to do so in the clone.